Актьори

• Drake Bell — Peter Parker / Spider-Man
• Caitlyn Taylor Love — Ava Ayala / White Tiger
• Ogie Banks — Luke Cage / Power Man
• Greg Cipes — Danny Rand / Iron Fist
• Logan Miller — Sam Alexander / Nova
• Chi McBride — Nick Fury
• Misty Lee — Doreen Green / Squirrel Girl
• Clark Gregg — Phil Coulson (voice)
• Scott Porter — Eugene 'Flash' Thompson / Agent Venom (voice)
• Tom Kenny — Otto Octavius / Doctor Octopus (voice)
• Donald Glover — Miles Morales / Kid Arachnid
• Misty Lee — Doreen Green / Squirrel Girl
• Matt Lanter — Harry Osborn / Venom / Anti-Venom / Patrioteer
• Phil LaMarr — Tyrone Johnson / Cloak
• Ashley Eckstein — Tandy Bowen / Dagger
• James Arnold Taylor — Triton
• Eric Bauza — Amadeus Cho / Iron Spider
• Tara Strong — Mary Jane Watson / Spider-Woman