Obsazení

• Tobey Maguire — Peter Parker / Spider-Man
• Kirsten Dunst — Mary Jane Watson
• James Franco — Harry Osborn
• Alfred Molina — Dr. Otto Octavius / Doctor Octopus
• Rosemary Harris — May Parker
• J.K. Simmons — J. Jonah Jameson
• Donnell Rawlings — Pizza 'Heist' Witness
• Joey Diaz — Train Passenger
• Christine Estabrook — Mrs. Jameson
• Louis Lombardi — Poker Player
• Elyse Dinh — Violinist
• Donna Murphy — Rosalie Octavius
• Elizabeth Banks — Miss Brant
• Daniel Gillies — John Jameson
• Dylan Baker — Dr. Curt Connors
• Willem Dafoe — Norman Osborn
• Bruce Campbell — Snooty Usher
• Stan Lee — Man Dodging Debris
• Joel McHale — Mr. Jacks
• Emily Deschanel — Receptionist
• Reed Diamond — Algernon
• John Paxton — Houseman
• Peter Allas — Train Passenger
• Roshon Fegan — Amazed Kid
• Hal Sparks — Elevator Passenger
• Kelly Connell — Dr. Isaacs
• Bill Calvert — Train Passenger
• Tom Carey — Train Conductor
• Tony Campisi — Train Passenger
• Susie Park — Clawing Nurse
• Mageina Tovah — Ursula
• Timothy Jerome — Injured Scientist
• Scott Spiegel — Man on Balcony
• Brendan Patrick Connor — Theater Traffic Cop
• Daniel Dae Kim — Raymond
• Aasif Mandvi — Mr. Aziz
• Vanessa Ferlito — Louise
• Joy Bryant — Woman at Web
• John Landis — Doctor
• Gregg Edelman — Dr. Davis
• Ted Raimi — Hoffman
• Cliff Robertson — Ben Parker
• Elya Baskin — Mr. Ditkovich
• Bill Nunn — Joseph 'Robbie' Robertson
• Brent Briscoe — Garbage Man
• Joanne Baron — Skeptical Scientist
• Peter McRobbie — OsCorp Representative
• Marc John Jefferies — Amazed Kid
• Brianna Brown — Train Passenger
• Molly Cheek — Society Woman
• Anne Betancourt — Woman at Fire
• Tricia Peters — Screaming Nurse