Ηθοποιοί

• Shameik Moore — Miles Morales
• Hailee Steinfeld — Gwen Stacy
• Jake Johnson — Peter B. Parker
• Oscar Isaac — Miguel O'Hara
• Issa Rae — Jessica Drew
• Jason Schwartzman — The Spot
• Brian Tyree Henry — Jeff Morales
• Luna Lauren Velez — Rio Morales
• Daniel Kaluuya — Hobie Brown
• Karan Soni — Pavitr Prabhakar
• Andy Samberg — Ben Reilly
• Rachel Dratch — Ms. Weber
• Shea Whigham — George Stacy
• Jorma Taccone — Adriano Tumino
• Taran Killam — Web-Slinger
• Nic Novicki — Lego Spider-Man
• Loraine Velez — Maria
• Peter Sohn — Ganke
• Atsuko Okatsuka — Yuri
• Nicole Delaney — MJ
• Ayo Edebiri — Glory
• Kathryn Hahn — Doc Ock
• Elizabeth Perkins — May
• Donald Glover — Aaron Davis
• J.K. Simmons — J. Jonah Jameson
• Ziggy Marley — Lenny
• Jack Quaid — Peter Parker
• Jharrel Jerome — Miles G. Morales
• Amandla Stenberg — Margo Kess
• Mahershala Ali — Uncle Aaron
• Greta Lee — LYLA
• Yuri Lowenthal — Insomniac Spider-Man
• Josh Keaton — Spectacular Spider-Man
• Metro Boomin — Metro Spider-Man
• Arthur Ortiz — Additional Voices (voice)
• Kimiko Glenn — Peni Parker
• Sofia Barclay — Malala Windsor