Näyttelijät

• Christopher Daniel Barnes — Peter Parker / Spider-Man
• Ed Asner — J. Jonah Jameson
• Sara Ballantine — Mary Jane Watson
• Gary Imhoff — Harry Osborn
• Jennifer Hale — Felicia Hardy
• Joseph Campanella — Dr. Curt Connors
• Julie Bennett — Aunt May Parker
• Linda Gary — Aunt May Parker
• Majel Barrett — Anna Watson
• Maxwell Caulfield — Alistair Smythe
• Rodney Saulsberry — Joseph "Robbie" Robertson
• Roscoe Lee Browne — Kingpin