Rollebesetning

• Paul Soles — Peter Parker / Spider-Man
• Paul Kligman — J. Jonah Jameson
• Peg Dixon — Betty Brant
• Len Carlson — Norman Osborn / Green Goblin
• Tom Harvey — Doctor Octopus / Dr. Otto Octavius
• Billie Mae Richards — Dr. Curtis Conners / Lizard Man
• Arthur Pierce — Max Dillon / Electro
• Ed McNamara — Aleksei Sytsevich / Rhino
• Claude Ray — Wilson Fisk / Kingpin
• Jack Mather — Flint Marko / Sandman
• Gillie Fenwick — Adrian Toomes / Vulture Man
• Chris Wiggins — Mysterio
• Bernard Cowen — Jackson Brice / Montana
• Carl Banas — Mac Gargan / Scorpion
• Henry Rammer — Spencer Smythe
• Max Ferguson — The Phantom
• Alfie Scopp — Captain Ned Stacy
• Janet Orenstein — Mary-Jane Watson
• June Foray — Aunt May Parker
• Larry D. Mann — Uncle Ben Parker
• Corinne Conley — Martha Conner