Rollista

• Danny Trejo — Machete
• Robert De Niro — Senator John McLaughlin
• Jessica Alba — Sartana Rivera
• Michelle Rodriguez — Luz
• Jeff Fahey — Michael Booth
• Steven Seagal — Rogelio Torrez
• Cheech Marin — Padre Cortez
• Don Johnson — Von Jackson
• Shea Whigham — Sniper
• Lindsay Lohan — April Booth
• Tom Savini — Osiris Amanpour
• Mayra Leal — Chica
• Jason Douglas — Patrolman #1
• Stacy Keach — Doc Franklin
• James Parks — Edgar McGraw
• Alicia Rachel Marek — June (as Alicia Marek)
• Electra Avellan — Nurse Mona
• Elise Avellan — Nurse Lisa
• Tina Rodriguez — Tristana
• Billy Blair — Von's Henchman
• Marci Madison — Nurse Fine
• Juan Gabriel Pareja — Rico
• Roland Ruiz — Luis
• Vic Trevino — Federale Officer
• Daryl Sabara — Julio
• Ara Celi — Reporter
• Cheryl Chin — Torrez's Henchwoman