بازیگران

• Oliver Platt — Dr. Mark Weller
• Paul Giamatti — Blaine Hammersmith
• Peter Boyle — Calloway
• Kyla Pratt — Maya Dolittle
• Jeffrey Tambor — Dr. Fish
• Steven Gilborn — Dr. Sam Litvack
• Chris Rock — Rodney the Guinea Pig
• Eddie Murphy — Dr. John Dolittle
• Ossie Davis — Archer Dolittle
• Richard Schiff — Dr. Gene Reiss
• Kristen Wilson — Lisa Dolittle
• Beth Grant — Woman
• Albert Brooks — Jacob "Jake" the Tiger
• Ellen DeGeneres — Prologue Dog
• Brian Doyle-Murray — Old Beagle (voice)
• Raven-Symoné — Charisse Dolittle