Cast

• Shameik Moore — Miles Morales / Spider-Man
• Hailee Steinfeld — Gwen Stacy / Spider-Woman
• Jake Johnson — Peter B. Parker / Spider-Man
• Kimiko Glenn — Peni Parker / SP//dr
• John Mulaney — Peter Porker / Spider-Ham
• Nicolas Cage — Peter Parker / Spider-Man Noir
• Mahershala Ali — Aaron Davis / Prowler
• Brian Tyree Henry — Jefferson Davis
• Chris Pine — Peter Parker / Spider-Man
• Kathryn Hahn — Olivia "Liv" Octavius / Doctor Octopus
• Liev Schreiber — Wilson Fisk / Kingpin
• Lake Bell — Vanessa Fisk
• Jorma Taccone — Norman Osborn / Green Goblin
• Lily Tomlin — May Parker
• Cliff Robertson — Ben Parker
• Marvin 'Krondon' Jones III — Tombstone
• Joaquín Cosío — Scorpion
• Stan Lee — Stan
• Post Malone — Brooklyn Bystander
• Natalie Morales — Miss Calleros
• Luna Lauren Velez — Rio Morales
• Zoë Kravitz — Mary Jane
• Arthur Ortiz — Additional Voices