演员

• Tobey Maguire — Peter Parker / Spider-Man
• Kirsten Dunst — Mary Jane Watson
• James Franco — Harry Osborn
• Willem Dafoe — Norman Osborn / Green Goblin
• J.K. Simmons — J. Jonah Jameson
• Cliff Robertson — Ben Parker
• Rosemary Harris — May Parker
• Joe Manganiello — Flash Thompson
• Gerry Becker — Maximilian Fargas
• Bill Nunn — Joseph 'Robbie' Robertson
• K. K. Dodds — Simkins
• Una Damon — Lab Tour Guide
• Michael Papajohn — Carjacker
• Elizabeth Banks — Betty Brant
• Octavia Spencer — Check-In Girl
• Lucy Lawless — Punk Rock Girl
• Bruce Campbell — Ring Announcer
• Ted Raimi — Hoffman
• Stan Lee — Man in Fair
• Jack Betts — Henry Balkan
• Stanley Anderson — General Slocum
• Sylva Kelegian — Mother at Fire
• Joseph D'Onofrio — Opinionated Cop
• Ashley Edner — Girl in Tram
• Scott Spiegel — Marine Cop
• Sara Ramírez — Cop at Carjacking
• Ron Perkins — Dr. Mendell Stromm
• Robert Kerman — Tugboat Captain
• Randall Poffo — Bone Saw McGraw
• Peter Appel — Cabbie
• Myk Watford — Cop at Fire
• Macy Gray — Herself
• Larry Joshua — Wrestling Promoter
• Kristen Marie Holly — Young Lady at Fire
• John Paxton — Houseman
• Jim Ward — Project Coordinator
• Jim Norton — Surly Truck Driver
• Jayce Bartok — Subway Guitarist
• Jason Padgett — Flash's Crony
• Evan Arnold — Doctor
• Deborah Wakeham — Billy's Mom
• Corey Mendell Parker — Chaperone in Tram
• Brad Grunberg — Heckler
• Bill Calvert — Fireman
• Alex Black — Boy in Tram
• Ajay Mehta — Cabbie