演员

• Tobey Maguire — Peter Parker / Spider-Man
• Kirsten Dunst — Mary Jane Watson
• James Franco — Harry Osborn / New Goblin
• Thomas Haden Church — Flint Marko / Sandman
• Topher Grace — Eddie Brock / Venom
• Bryce Dallas Howard — Gwen Stacy
• Rosemary Harris — May Parker
• J.K. Simmons — J. Jonah Jameson
• James Cromwell — Captain Stacey
• Theresa Russell — Mrs. Marko
• Dylan Baker — Dr. Curt Connors
• Bill Nunn — Joseph Robertson
• Bruce Campbell — Maître d
• Elizabeth Banks — Miss Brant
• Ted Raimi — Hoffman
• Willem Dafoe — Norman Osborn / Green Goblin
• Cliff Robertson — Ben Parker
• Perla Haney-Jardine — Penny Marko
• Elya Baskin — Mr. Ditkovitch
• Joe Manganiello — Flash Thompson
• John Paxton — Houseman
• Stan Lee — Man in Times Square
• Marc Vann — Play Producer
• Reynaldo Gallegos — Armored Car Driver
• April Parker Jones — Test Site Technician
• Emilio Rivera — Policeman at Sand Truck
• Mageina Tovah — Ursula
• Michael Papajohn — Dennis Carradine / Carjacker
• Becky Ann Baker — Mrs. Stacy
• Lucy Gordon — Jennifer Dugan
• Steve Valentine — Photographer
• Tim Maculan — Play Director
• Joe Bays — Jazz Club Manager
• Gregg Daniel — Precinct Detective
• Rogelio T. Ramos — Emergency Room Doctor
• Edward Padilla — Test Site Technician
• Robert Curtis Brown — Test Site Technician
• Paul Terrell Clayton — Test Site Technician
• Christina Cindrich — Test Site Technician
• Sonya Maddox — ICU Nurse
• Toni Wynne — Congratulatory Woman at Daily Bugle
• Michael Alexander — New Jersey State Policeman
• Jim Coope — Newstand Patron
• Dean Edwards — Newstand Patron
• Aimee Miles — Coffee Shop Waitress
• Mark Kubr — Jazz Club Bouncer
• Christopher Young — Pianist
• Daniel Gillies — John Jameson